Signalling

Part:BBa_K2924050:Design

Designed by: Melanie Sbielut   Group: iGEM19_Duesseldorf   (2019-10-15)


SPYurl


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

SPYurl is a signal peptide used in the PBSMul1 plasmid to express heterologous proteins in B. subtilis and secrete them into the culture supernatant.

Source

Taken from the PBSMul1 plasmid, provided by Dr. Andreas Knapp from the research center Jülich1.

References

[1]: Knapp, A., Ripphahn, M., Volkenborn, K., Skocinski, P., Jaeger, K. E. (2017). "Activity-independent screening of secreted proteins using split GFP." J Biotechnol 258: 110-116.